Circulating tumor DNA for monitoring colorectal cancer: A prospective observational study to assess the presence of methylated SEPT9 and VIM promoter genes and its role as a biomarker in colorectal cancer management

Objective: Methylation status of Septin9 (SEPT9) and vimentin (VIM) genes in circulating tumor DNA colorectal cancer (CRC) patients is a promising bio-marker for the early detection CRC. The aim present study was to identify methylation promoter regions SEPT9 VIM cohort Indian with biopsy proven cancer. Material Methods: Forty-five consecutive were recruited. 10 mL venous samples collected from each patient processed isolation cell-free DNA, bisulfite conversion polymerase chain reaction (PCR) amplification genes. Results: Partial 42.22% 57.78% showed no none tumors had complete methylation. Only three (6.66%) patterns remaining 42 (93.33%) partial Considering two together, only out 45 association both (complete, partial, methylation) sex, age, T stage, N M CEA, histology, location (right or left colon) explored these parameters statistically significant. Conclusion: In our study, 6.66% CRC hypermethylation there any like TNM histology.